Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: Diagnostic profile of clinical images, histopathological findings, Ziehl-Neelsen staining, and DNA microarray chip assay of participants with cutaneous mycobacterial infections. (A-C) Skin lesions of participants with cutaneous infection of M. marinum (A) , M. abscessus (B) , MTB (C) , and M. chelonae (D) . (E, F) Hematoxylin and eosin staining revealing acanthosis, pseudoepitheliomatous hyperplasia or exocytosis, diffuse inflammatory cells infiltration in the dermis, including multinucleated giant cells (white arrow), lymphocytes, neutrophils, and plasmocytes (magnification: A, 40×; B, 200×). (G) Ziehl-Neelsen staining of isolates from skin tissue culture showing red club-shaped filaments. (H-K) Mapped images of the DNA microarray chip assay of skin tissue confirming infection with M. marinum (H) , M. abscessus (I) , MTB (J) , and M. chelonae (K) .

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Diagnostic Assay, Staining, Microarray, Infection

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: Sensitivities, specificity and accuracy of the skin culture method and DNA microarray chip assay in the diagnosis of cutaneous mycobacterial infections.

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Microarray, Biomarker Discovery

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: PLR, NLR, Youden’s index and OR of the skin tissue culture method and DNA microarray chip assay.

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Microarray

Journal: BMC Microbiology

Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

doi: 10.1186/s12866-020-01840-5

Figure Lengend Snippet: Non-tuberculous species identified from 2013 to 2018 in a tertiary hospital in Beijing, China

Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

Techniques:

Journal: BMC Microbiology

Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

doi: 10.1186/s12866-020-01840-5

Figure Lengend Snippet: Specimen types among which NTM were identified in this study

Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

Techniques:

Journal: BMC Infectious Diseases

Article Title: Rapid and accurate detection of RMP- and INH- resistant Mycobacterium tuberculosis in spinal tuberculosis specimens by CapitalBio™ DNA microarray: A prospective validation study

doi: 10.1186/1471-2334-12-303

Figure Lengend Snippet: Overall sensitivity of the DNA microarray for the M. tuberculosis strains identification

Article Snippet: The CapitalBio™ DNA microarray achieved 93.55% sensitivity for the correct M. tuberculosis species identification of the 93 specimens that tested positive for spinal tuberculosis through culture.

Techniques: Microarray

Journal: BMC Infectious Diseases

Article Title: Rapid and accurate detection of RMP- and INH- resistant Mycobacterium tuberculosis in spinal tuberculosis specimens by CapitalBio™ DNA microarray: A prospective validation study

doi: 10.1186/1471-2334-12-303

Figure Lengend Snippet: Hybridization pattern of spinal tuberculosis specimens with the DNA microarray. a . Hybridization pattern of the M. tuberculosis strain identification (white frame) b . RMP resistance with the rpoB 531 (T C G->T T G) mutation, the hybridization signal of probe TCG531TTG (solid rectangle) being higher than corresponding wild-type probe (dashed rectangle) c . RMP resistance test: rpoB 516 (G A C->G T C) mutation, the hybridization signal of probe GAC516GTC (solid rectangle) being higher than corresponding wild-type probe (dashed rectangle) d , e . INH resistance test: katG 315 (A G C->A C C) mutation, the hybridisation signal of probe katG AGC315ACC (solid rectangles) were higher than corresponding wild-type probe (dashed rectangles) e. INH resistance test: inhA -15 C-T mutation, the hybridisation signal of probe inhA 15C→T (solid rectangles) were higher than corresponding wild-type probe (dashed rectangles).

Article Snippet: The CapitalBio™ DNA microarray achieved 93.55% sensitivity for the correct M. tuberculosis species identification of the 93 specimens that tested positive for spinal tuberculosis through culture.

Techniques: Hybridization, Microarray, Mutagenesis

Journal: BMC Infectious Diseases

Article Title: Rapid and accurate detection of RMP- and INH- resistant Mycobacterium tuberculosis in spinal tuberculosis specimens by CapitalBio™ DNA microarray: A prospective validation study

doi: 10.1186/1471-2334-12-303

Figure Lengend Snippet: Performance of the DNA microarray assay for detection of resistance to INH and RMP compared with phenotypic DST

Article Snippet: The CapitalBio™ DNA microarray achieved 93.55% sensitivity for the correct M. tuberculosis species identification of the 93 specimens that tested positive for spinal tuberculosis through culture.

Techniques: Microarray

Journal: BMC Microbiology

Article Title: Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

doi: 10.1186/s12866-021-02246-7

Figure Lengend Snippet: Evaluation and comparison of drug resistance rate of anti- tuberculosis drugs in 2014–2019

Article Snippet: This study was based on M. tuberculosis drug resistance gene detection kit (CapitalBioTM DNA microarray method, Beijing CapitalBio Technology, 301,035), which can specifically detect the mutations of rpoB , katG and inhA .

Techniques: Comparison

Journal: BMC Microbiology

Article Title: Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

doi: 10.1186/s12866-021-02246-7

Figure Lengend Snippet: Trends of different drug-resistance patterns among 1484 culture-confirmed TB cases in Hainan, 2014 to 2019. In new cases, for INH resistance (χ 2 = 2.813, P = 0.729); for RIF resistance (χ 2 = 3.181, P = 0.672); for MDR-TB (χ 2 = 4.210, P = 0.520); for XDR-TB (χ 2 = 4.383, P = 0.496). In retreatment cases, for INH resistance (χ 2 = 9.512, P = 0.090); for RIF resistance (χ 2 = 14.257, P = 0.014); for MDR-TB (χ 2 = 10.328, P = 0.066); for XDR-TB (χ 2 = 7.670, P = 0.175). Note: *new cases, **retreatment cases. Abbreviation: INH-R, isoniazid resistance; RIF-R, rifampin resistance; MDR-TB, multidrug resistant tuberculosis; XDR-TB, extensively drug resistant tuberculosis

Article Snippet: This study was based on M. tuberculosis drug resistance gene detection kit (CapitalBioTM DNA microarray method, Beijing CapitalBio Technology, 301,035), which can specifically detect the mutations of rpoB , katG and inhA .

Techniques:

Journal: BMC Microbiology

Article Title: Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

doi: 10.1186/s12866-021-02246-7

Figure Lengend Snippet: Evolution of drug resistance mutation sites of Mycobacterium tuberculosis to first-line anti tuberculosis drugs isoniazid and rifampin in 2014–2019

Article Snippet: This study was based on M. tuberculosis drug resistance gene detection kit (CapitalBioTM DNA microarray method, Beijing CapitalBio Technology, 301,035), which can specifically detect the mutations of rpoB , katG and inhA .

Techniques: Mutagenesis

Journal: BMC Ophthalmology

Article Title: MiRNAs regulate oxidative stress related genes via binding to the 3′ UTR and TATA-box regions: a new hypothesis for cataract pathogenesis

doi: 10.1186/s12886-017-0537-9

Figure Lengend Snippet: Heatmap shows differentially expressed mRNAs in cataractous lens samples compared with transparent lens samples. Six separate microarray assays were performed to determine the genome-wide mRNA expression in the central epithelium of transparent and cataractous human lenses. Microarray data were processed by CapitalBio Corporation. Heatmap shows differentially expressed mRNAs in cataractous lens samples compared with transparent lens samples. Relative expression value from high to low was shown by gradient of red to green in the heatmap. Colors indicate relative mRNA expression. Red and green indicate higher or lower expression of mRNAs relative to those in transparent lens samples, respectively. FDR (false discovery rate) adjusted p -value <0.05

Article Snippet: Gene Chip microarray service of CapitalBio Corporation is certificated by Affymetrix.

Techniques: Microarray, Genome Wide, Expressing